动物造模,小鼠肺部炎症 z-bio 2021-07-22 550

　　Purpose: TNF-α in the serum of mice infected with Mycoplasma pneumonia (MP)? INF-γ content and MPN372? P1? To explore the effect of Qingzao Jiufei Decoction (QJD) and its degradation products on lung tissues expressing AQP5, the molecular mechanism of MP infection reveals its drug resistance?

　　Method: 144 SPF BABL/c A group of random mice (group A)? Model group (group B)? QJD Group (Group C)? QJD disintegrant I group (D group) QJD disintegrant II group (E group) and azithromycin group (F group), 24 in each group? Except for group A, the other 5 groups of mice were treated with MP infection model. After modeling, the corresponding group of drugs were given, and samples were taken on the 3rd, 7th, 10th, and 14th days after modeling. Observe pathological sections of mouse lung tissue under a microscope, record the degree of pathological inflammation in mouse lung tissue, and calculate mouse lung index and dry humidity. Comparison; Does MPN372 use qPCR method? Determine the P1 gene content; enzyme-linked immunosorbent assay (ELISA) in each group of mice serum TNF-α? Measure changes in INF-γ levels; Western blotting method for detecting AQP5 protein expression?

　　Result: After MP infection, thickening of the alveolar septum can be confirmed under the microscope. , The bronchial wall is thickened and a large number of inflammatory cells are infiltrated; the lung index increases, and the dry-to-wet ratio decreases; TNF-α content? It reached a peak on the 7th day; the expression of protein AQP5 showed a downward trend and gradually increased on the 14th day. Compared with group B, from the 7th day, group C is MPN372? We can down-regulate the expression levels of P1 and TNF-α. -Adjust INF-γ? The expression level of AQP5; among them, the role of group D is mainly MPN372? P1? Is it down-regulating the expression of TNF-α, and the effect of group E is mainly up-regulating INF-γ? AQP5?

　　Conclusion: QJD regulates lung inflammation after MP infection, reduces the production of MP toxin MPN372 and the expression of P1 adhesion protein INF-γ? The expression of AQP5 protein can be up-regulated and down-regulated—is regulating the expression of TNF-α one of its mechanisms of action?