型糖尿病肾病大鼠模型,动物造模 z-bio 2021-07-22 565

　　Purpose: How to study the effect of different high-fat diet feeding time on blood glucose, insulin resistance index (HOMA-IR) and urinary albumin excretion rate (UAER) in type 2 diabetic nephropathy (DN) rat model?

　　Method: Use unilateral renal artery ligation + high-fat diet feeding + low-dose streptozotocin (STZ) intraperitoneal injection to create a model. After the unilateral renal artery was ligated, the DN1 and DN2 groups were fed a high-fat diet 4 times. These are weeks or 8 weeks, respectively. STZ 30 mg/kg was intraperitoneally injected 4 weeks later, and the UAERs of the two groups of rats were compared 4 weeks after STZ injection and at the end of the experiment. Blood glucose, body weight, HOMA-IR, kidney index, kidney pathology of the two groups of rats

　　Result: 4 weeks after STZ injection, the UAER of the two groups of rats may increase. The UAER of the DN2 group was significantly higher than that of the DN1 group (P0.05)?

　　Conclusion: Increasing the feeding time of a high-fat diet may exacerbate DN kidney damage, but should the study consider options because it may delay the intervention time?