动物造模,过敏性结膜炎小鼠模 z-bio 2021-07-26 614

　　Objective: To establish mouse models of allergic conjunctivitis under different antigens, different excitatory pathways, and different ages, to compare the immune response status, to experience the best mouse models of allergic conjunctivitis, and to screen crop conditions. Establish and provide new ideas and methods.

　　Methods 65 SPF Balb/c mice, different antigens (yolk protein, ragweed pollen, mice), different excitatory routes (forced feeding, spraying, subcutaneous injection), different mouse ages (1 week, 2 weeks, 3 weeks old) ) An animal model of allergic conjunctivitis was established at 8 weeks of age). T-qPCR was used to detect the relative expression levels of CCL5 mRNA and IL-17 mRNA in mouse conjunctival tissues. Flow cytometry was used to detect CD4+ T cells in the splenic single cell suspension. It has been detected that -17? and

　　Compared with the negative control group, the expression rates of IL-17, CCL5 mRNA, and IL-17 mRNA in the egg white albumin group, house dust mite group, and ragweed pollen group all increased, and the IL-17, CCL5 mRNA, CCL5 mRNA, and IL-17 mRNA in the ragweed pollen group The expression rate of IL-17 mRNA increased. the most important. get. Compared with the negative control group, the expression ratio of IL-17 and IL-17 mRNA in the subcutaneous injection group, gavage group, spray inhalation group and positive control group increased, and the expression of CCL5 mRNA in the gavage group was similar. used to be. Negative control group. The expression level of this group was close to that of the positive control group. The percentages of IL-17, CCL5 mRNA, and IL-17 mRNA expression levels in 1-week and 2-week-old mice were similar, but significantly lower than 3- and 8-week-old mice. ..

　　Conclusion Ragweed pollen has good allergenicity in establishing animal models of allergic conjunctivitis in mice. Subcutaneous injection of stimulating antigen can achieve satisfactory results in the structure of mouse animal model of allergic conjunctivitis. There are no obvious eye symptoms or signs, but the body and parts are sensitive. The immune response of 3-week-old Balb/c mice is similar to that of adult Balb/c mice.