动物造模,2型糖尿病模型 z-bio 2021-07-26 357

　　Objective: To explore how to prepare a rat model of diabetes induced by high-fat diet combined with streptozotocin (STZ), and provide a stable animal model of diabetes for clinical research.

　　Methods: Thirty male SD rats were randomly divided into two groups: normal diet fed normal control group (NG, n=10); high fat diet (HFD)-feeding type 2 diabetes group (DM, n=20) for weeks + STZ 25 mg/kg was injected through the tail vein. During the experiment, the rats were weighed every week to observe the changes in the area under the blood glucose curve in the oral glucose tolerance test (OGTT), insulin tolerance test (ITT) and one week before STZ injection. After STZ injection, observe the general conditions of the rats (food intake, water consumption, hair gloss, bedding moisture, mental status), and randomly detect the blood glucose level and fruit amine of the rats.

　　Result: Compared with the NG group, the weight of the DM group increased significantly, and the difference was significant after 12 weeks of feeding (Pu003c0.05). OGTT experiment 60, 90, 120 minutes blood glucose level was significantly higher than the NG group (Pu003c0.05), the area under the curve increased significantly (Pu003c0.05); the value was significantly higher than the NG group (Pu003c 0.05), the area under the curve Significant increase (Pu003c0.05). After STZ intervention, there were significant differences between the two groups of rats in food intake, water intake, body weight, etc. (Pu003c0.05). The random blood glucose and fructosamine of the DM group were significantly higher than those of the NG group (Pu003c0.05).

　　Conclusion: High-fat diet rats cause insulin resistance and impaired glucose tolerance after 12 weeks. The type 2 diabetic rat model with stable blood glucose level induced by low-dose STZ is an ideal animal model for studying type 2 diabetes.