动物造模,慢性心肌缺血模型 z-bio 2021-11-06 570

　　OBJECTIVE: To establish a mouse chronic myocardial ischemia model by subcutaneously injecting different doses of isoproterenol (ISO) into the neck, determine the best model dose through comparison, and refer to the preparation of related models to find more objective and reliable indicators for evaluation. supply. Efficacy evaluation.

　　Method: The three model groups were injected subcutaneously with ISO 32mg/kg, 16m/kg and 8mg/kg body weight for 3 consecutive days. An electrocardiogram (ECG) was recorded 24 hours, 8 days, 15 days, and 8 days after the model was created. Collect the myocardial injury markers and heart specimens of each group to observe the pathological changes of myocardial morphology in each group.

　　Results: The T wave of the ECG in the ISO 32 m/kg and ISO 16 m/kg groups was significantly reduced at 8 and 15 days after modeling (Pu003c0.05 or Pu003c0.01). Markers of myocardial injury changed significantly (Pu003c0.05). Or Pu003c0.01)), if the ISO32m/kg group has pathological changes, the mortality rate is 42.5? This is more suitable for evaluating the long-term efficacy of anti-chronic myocardial ischemia drugs. .. ISO 16 mg/kg has a relatively low mortality rate of 25? and does not cause pathological damage to the myocardium.

　　Conclusion: According to various experimental expectations, the ISO 16 or 32 mg/kg model dose can be selectively used to establish a mouse chronic myocardial ischemia model.