动物造模,帕金森模型 z-bio 2021-09-23 678

　　Objective: To establish a stable 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced C57BL/6 mouse Parkinson's model and evaluate its behavior.

　　"Establishment": The method of administration group, the doses are 35mg/kg, 30mg/kg, 25mg/kg, and the control group is given 30mg/kg normal saline. Carry out tremor paralysis score, field test, pole climbing test, suspension test, swimming test and comprehensive statistics. The differences between the treatment group and the control group and between the treatment groups are analyzed. At the same time, HE and immunohistochemical TH antibody staining were performed to observe the changes of neurons and nerve fibers in the substantia nigra and striatum.

　　Result: Compared with the control group, the score of the treatment group was significantly higher than that of the control group, but the behavior score of the 25mg/kg group was significantly lower than that of the 35mg/kg and 30mg/kg groups. Significant differences. The number of TH-positive neurons in the substantia nigra decreases, and the number of TH-positive nerve fibers in the striatum decreases.

　　Conclusion: A simple method has been established to evaluate the efficacy of mouse Parkinson's disease models through behavioral data.