动物造模,兔膝骨关节炎模型 z-bio 2021-07-27 449

　　Objective: To establish a rabbit knee osteoarthritis model and evaluate it through rabbit cartilage defects.

　　Method: The animals were randomly divided into 3 groups according to body weight and sex. There were 12 rats in the sham operation group, model group and positive control glucosamine group. After 4 weeks of modeling, the stomach was given once a day for 4 consecutive weeks. After the last administration, at the expense of animals, the levels of IL-1β and TNF-α in serum and synovial fluid were detected by ELISA kit, joints and synovium were stained with HE, and Makin damage of articular cartilage was evaluated. Increase.

　　Result: Compared with the sham operation group, the IL-1β and TNF-a levels in the serum and synovial fluid of the model group were significantly increased. Glucosamine can reduce serum IL-1β and TNF-a levels. Synovial fluid. HE staining showed changes in cartilage structure and defects in the model group, infiltration of synovial inflammatory cells, and cartilage scores were significantly higher than those in the sham operation group. Glucosamine can reduce cartilage defects, reduce synovitis cell infiltration, and lower cartilage scores.

　　Conclusion: The articular cartilage drilling reflects its key feature of establishing a rabbit knee osteoarthritis model, and it can reduce injury and inflammation by giving a positive control. This model can be used to evaluate and screen drugs for the treatment of arthritis.