动物造模,肝纤维化模型 z-bio 2021-07-27 902

　　Objective: To compare the induction effects of different doses of carbon tetrachloride on the self-bred C57BL/6 mouse liver cirrhosis model, and to establish a stable liver cirrhosis model.

　　Method: Choose 5-week-old C57BL/6 inbred mice, and intraperitoneally inject high, medium and low doses of carbon tetrachloride to induce liver cirrhosis. After the experiment is completed, blood and liver tissue will be collected and plasma measured. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and liver pathological changes.

　　Result: After self-bred C57BL/6 mice were induced by drugs, the animals in each dose group can survive for up to 8 weeks, and the survival rate of the high-dose group is only 20? much lower than that of the middle-dose group. In the low group, the serum transaminase level increased, and the pathological changes of liver tissue had a dose-time effect relationship. Pathological analysis showed that hepatocyte degeneration/necrosis, mixed cell infiltration around central vein/portal area, and liver fibrosis occurred in each dose group.

　　Conclusion: 8 weeks after the administration of 10? carbon chloride, the allogeneic C57BL/6 mice were induced to form a relatively stable mouse model of liver fibrosis, and then the mechanism of liver fibrosis was studied to lay the foundation for drug screening.