动物造模,压力超负荷心衰小鼠模型 z-bio 2021-07-27 681

　　Objective: To establish a mouse model of heart failure, using ultrasound images and pathological techniques to comprehensively evaluate the characteristic changes in cardiac function and structure during the onset of disease.

　　Method: Use lateral aortic stenosis (TAC) surgical technique to establish a pressure-overload heart failure model in mice, 0 weeks before the model, 2, 4, aortic arch stenosis blood flow Doppler. collect. 8 weeks after modeling. Estimate vascular pressure and collect B-mode and M-mode ultrasound images to evaluate the left ventricular systolic function of the heart structure, and combine the mitral valve annulus to collect the mitral annulus blood flow Doppler.. Used to evaluate the expansion function and collect the heart tissue Tissue Doppler. Histopathological observation.

　　Results: Ultrasound results showed that the aortic blood pressure increased significantly at 2, 4, and 8 weeks after surgery. Two weeks after the operation, the main symptoms were a characteristic compensatory increase in left ventricular wall thickness, decrease in inner diameter, and enhanced compensatory systolic function, but accompanied by impaired diastolic function and myocardial fibrosis. Four weeks after the operation is a transitional period. This is mainly manifested as the transition from ventricular wall hypertrophy to ventricular expansion, systolic function begins to decline, diastolic function continues to be impaired, and the degree of myocardial fibrosis increases; 8 weeks after surgery, the characteristic ventricular space expands, and systolic and systolic blood pressure are significantly reduced. Contraction and relaxation function. The cardiomyocytes were partially lysed and obviously fibrotic.

　　Conclusion: By evaluating the structure and function of the left ventricle in the three stages of compensatory myocardial hypertrophy, mouse aortic arch contraction transforms to decompensated dilated heart failure. The basic and transformational model research of aortic arch contraction provides a theory. Base.