动物造模,盆腔脏器脱垂动物模型 z-bio 2021-07-27 421

　　Objective: To establish a rat animal model that can reflect the similar pathological changes of the sacral ligament in patients with pelvic organ prolapse, and to explore the effects of increased labor intensity and simulated menopause on the pathological changes of the rat's sacral ligament.

　　Method: Select 60 adult SPF-level SD female rats, weighing about 300g, of which 45 have no birth history, and simulate according to the blank control group (A), simulated menopausal group (B), and random digital table. It is divided into birth injuries. Methods: In the simulated menopause group (C), the other 15 infants who gave birth 3 times in a row were birth injuries + simulated birth injuries + simulated menopause group (D). Group A had a normal diet, group B had bilateral ovaries removed, group C had simulated birth injuries on both sides of the ovaries to simulate menopause, group D had 3 consecutive ovaries removed, and I had 2 simulated births on both sides after delivery. Ovariectomy simulates menopause. They reproduce regularly within 8-10 weeks after surgery. Eight rats were randomly selected from each group to observe the changes in the appearance and dormancy of the genitals of the rats in each group. Immunohistochemical methods are used to observe and evaluate the type I and type III collagen in the front wall of the vagina. (COL1A1, COL3A1), transforming growth factor β-3 (TGFβ-3) comprehensive optical density changes. T-PCR is used to detect the expression levels of COL1A1, COL3A1 and TGFβ-3 mRNA in the sacral ligament tissue.

　　Result: After modeling, the diameter of the vaginal slit in the D group was larger than that of the control group by more than 2 mm. Rats in each group had no obvious prolapse phenotype, and mild perineal abnormalities appeared in group D. The expression of wall COL1A1 was significantly lower than that of group A (Pu003c0.05). Compared with group A, the relative expression of TGFβ-3 in group C and group D was significantly higher than that in group A (P\u003c0.05). The expression of COLIA1 and COL3A1 in the sacral ligament of D rat was significantly lower than that of group A (Pu003c0.05). Compared with group A, the relative expression of TGFβ-3 in group C and group D was significantly higher than that in group A (P\u003c0.05). ).

　　Conclusion: The phenotype of the rat model after modeling, such as the study of phenotypic changes, is not important as the research content, and it is not appropriate to establish this model. This model can be used as an animal to study pathophysiology. It is a modification of the POP sacral ligament model, but it cannot be determined whether the mechanical properties of the human and rat sacral ligaments are similar. The best animal model should be selected according to the strengths and weaknesses of each different animal and actual research.