动物造模,门脉高压动物模型 z-bio 2021-07-27 434

　　Objective: To investigate whether a simple ligation of the left portal vein can form a stable portal hypertension, and whether nitric oxide plays a corresponding role.

　　Method: 36 SPF male 8-week-old SD rats were randomly divided into left branch ligation group (needle diameter 0.6, 0.7, 0.8 mm) and main ligation group (needle diameter 0.9 mm). There are 6 groups of ligation), left lateral lobectomy group and sham operation group. The portal pressure of all rats was measured before surgery, immediately after surgery, 15 days after surgery, and 30 days after surgery. All rats were sacrificed on the 30th day after surgery, and portal vein blood and left and right liver tissues were collected to determine the content of nitric oxide (NO, NO, NO).

　　Results: The portal vein pressure of SD rats in the left branch ligation group immediately after surgery, 15 days and 30 days after surgery were significantly higher than that before surgery and the control group. There was no significant difference in the left branch ligation group of 0.6 mm. There was no statistical difference in NO content in the left and right liver tissues and blood of the 6 groups of SD rats.

　　Conclusion: Simple ligation of the left portal vein can form stable portal hypertension, and a 0.6mm diameter needle can achieve better results.