动物造模,冠心病气虚血瘀证病证结合大鼠模型 z-bio 2021-07-27 855

　　Objective: To establish a compound model of the relationship between pathology, etiology, coronary heart disease (CHD) Qi deficiency and blood stasis syndrome combined with tongue symptoms and the relationship between prostacyclin (PGI2) and thromboxane A2 (TXA2) in rats.

　　Methods: 32 rats were randomly divided into 4 groups: group K: normal diet and drinking (n=8); group L: normal feeding for 21 days and ligation of the left anterior descending branch (n=) 8); group Y: 21 rats- Daily food management compound fatigue exercise (n = 8); Group F: 21 days food management compound fatigue exercise + left anterior descending artery ligation (n = 8). After the modeling is completed, Photoshop 6.0 is used to analyze the RGB value of the tongue surface, and the RGB data distribution range table is used to evaluate the tongue color of each model. HE staining detects filamentous papillary epithelial layer, corneal layer, lamina propria height and lamina propria height. The number of capillaries in the lamina propria; immunohistochemical analysis of the positive expression of PGI2 and TXA2 and calculation of the T/P ratio.

　　Result: (1) The tongue quality of group K was caused by "bright red tongue". Due to the "darkness", the R value of the tongue surface of the rats in the L group was significantly lower than that of the rat tongue in the K group. "Red tongue"; the R value of the tongue surface of the rats in the Y group was significantly lower than that of the K group, belonging to the "light tongue"; the R, G, and B values of the tongue surface of the rats in the F group were respectively in the K group and were significantly lower than the L group, which belonged to " Purple tongue". (2) The height of each layer of the lingual filamentous papilla and the number of microvessels in the L group were not different from those in the K group. The height of the filiform papillary keratinization layer in group Y and K was significantly lower; the height of filiform papillae epithelium and keratinization layer in group F was significantly lower. Significantly higher than group K and group L; no difference (3) PGI2 changes in group L, Y, and F were not significant compared with group K; group L TXA2 level and T/P ratio were significantly higher than group K; group F The TXA2 level and T/P ratio were significantly higher than those in the K and L groups.

　　Conclusion: The RGB characteristics of tongue color in the syndrome of qi deficiency and blood congestion in coronary heart disease are related to the decrease in the height of the filamentous papillary epithelium and stratum corneum, and the increase in the number of capillaries in the lamina propria. Its molecular biological mechanism may be related to the imbalance of TXA2/PGI2. Adopt pathology. The combined pathogenesis model is more in line with the characteristics of the congenital heart disease model, combining deficiency syndrome and congestion syndrome.