动物造模,慢性血栓小鼠模型 z-bio 2021-07-28 786

　　Objective: To treat mice by continuous intraperitoneal injection of different concentrations of carrageenan solution, and to screen the disease state of the mouse model of chronic thrombosis.

　　Method: 70 ICR male mice were randomly divided into 7 groups, 10 in each group. Namely the blank control group, 0.02-meter group, 0.04-meter group, 0.06-meter group, 0.08-meter group, 0.10-meter group, and 0.20-meter group. The mice in the blank group were injected with normal saline (0.01 mL/g) into the abdominal cavity, and the mice in the other groups were injected with the corresponding concentration of carrageenan solution (0.01 mL/g) once a day. ...Raise each group of mice. Eat and drink freely at room temperature (20±1). Observe the occurrence of black tails in each group (percentage of black tails, length of black tails) every day. Take a sample when a blood clot has formed. Blood was drawn to detect four types of coagulation items, as well as oxidative stress and inflammatory factors in colon tissue. And test the plasma.

　　Result: 0.06 mice had 2 thrombus on the 5th day, and 8 mice on the 6th day. Compared with the blank control group, the FIB content of mice in the 0.06? drug group was significantly increased (Pu003c0.05), APTT was significantly shortened (Pu003c0.05), and TT was significantly prolonged (Pu003c0.05) u003c0.05).Pu003c0. 05), PT was significantly prolonged (Pu003c0.01); SOD and GSH-Px in colon tissue and plasma in the 0.06? dose group were significantly reduced (Pu003c0.01), and MDA in the 0.06? dose group was significantly increased (Pu003c0.05) , TNF-α and IIIL-1β in colon tissue and plasma were significantly increased (Pu003c0.05), and IL-10 was significantly increased (Pu003c0.05).

　　Conclusion: It is preliminarily concluded that the continuous intraperitoneal injection of 0.06? Betanic acid solution (0.01 mL/g) at room temperature (20±1) ℃ for 6 days (once a day) is most suitable for establishing mice. Model of chronic thrombotic conditions.