动物造模,类风湿关节炎动物模型 z-bio 2021-07-28 408

　　Objective: To study the modeling elements of animal models of rheumatoid arthritis, improve the success rate of modeling, and provide methodological references for evaluating the efficacy of tested drugs.

　　Methods: Using rheumatoid arthritis and animal models as the subject terms, search the Chinese Journal Full-text Database (CNKI) and Wanfang Database (January 2017-June 2020) related journal articles, and establish a database of experimental animal types and excitation methods , Inflammation collection methods, detection indicators, etc., and use Excel 2013 and SPSSStatistics19.0 statistical software to conduct association rule analysis and factor analysis on the imported detection indicators. ...

　　Result: included in 274 journal articles that meet the criteria. Among them, the most used experimental animals are male SD rats (69 times, 25.18? and male Wistar rats (54 times, 19.71?. The methods that cause inflammation include the CIA method (172 times, 62.77? and AA method (82 times, 29.93?. The most commonly used method of excitement is subcutaneous injection at the base of the tail (71 times, 25.91?. Intradermal injection on the right hind paw (52 times, 18.98?.) The most detected index is plantar swelling (133 times, 17.48?, arthritis score Index (84 times, 11.04? and serum TNF-α. Level (74 times, 9.72? and joint histopathology (66 times, 8.67?, etc.).

　　Conclusion: When modeling the animal model of rheumatoid arthritis, use male SD rats or male Wistar rats, subcutaneous injection of the tail root causes inflammation and incomplete bovine type II collagen and Freund's or complete adjuvant 1: 0.1mL per point mixed One lotion can cause inflammation and increase the success rate of the model. After browsing the literature in the past three years, a total of 274 documents meeting the requirements were screened out. This article analyzes the existing animal models and proposes further improvement of rheumatoid arthritis animal models and standardization of model evaluation. Improve consistency between animal models and clinical practice. The research progress of rheumatoid arthritis animal models is summarized from the aspects of model animal selection, rheumatoid arthritis model types, modeling agents, detection indicators, etc., which provides convenience for in-depth research on rheumatoid arthritis.