动物造模,移植模型 z-bio 2021-07-28 421

　　Objective: To establish a rat xenotransplantation model of human glioma using immunosuppressive agents for the first time to provide an ideal experimental tool for clinical and basic research.

　　Methods: Clinically commonly used immunosuppressants rapamycin (rapamycin) and cyclosporine A (cyclosporine (A), SD rats start compulsory feeding 3 days before surgery, rapamycin single agent (Rapa) group, ring Single-drug selection method for spores (CsA)) Human sirolimus U87-MG cells were used for orthotopic brain transplantation and right back of SD rats, respectively. The tumor volume and body weight were measured by subcutaneous transplantation, and the changes were monitored. When the tumor grows to about 60 omm3, the growth of rat glioma in situ is detected by near-infrared fluorescence in vivo imaging, and then tissue specimens are separated at the expense of rats. Use HE staining and immunohistochemistry to detect tumors. Organizational form.

　　Results: Compared with the Rapa and CsA groups, the combination therapy (Rapa+CsA) showed obvious advantages in immunosuppression, the model success rate reached 100? Rapa and CsA single-agent modeling), the success rate was 0? The combination therapy group was greater The body weight of the rats was significantly smaller than the Lapa group, the CsA group and the control group. In vivo imaging showed that the rat brain was rich in near-infrared fluorescent dyes. Histopathological analysis confirmed that it was a glioma. Human mitochondrial mitochondrial antibody immunohistochemical staining showed a strong positive tumor site. As shown

　　Conclusion: The combination of immunosuppressive drugs and inoculation of human glioma U87-MG cells (Rapa+CsA) can successfully establish a rat allogeneic transplantation model of human glioma. Pathological analysis confirmed the histological morphology of human glioma.