动物造模,慢性高尿酸血症肾损害大鼠模型 z-bio 2021-07-28 636

　　Objective: To establish a rat model of chronic hyperuricemia nephropathy and provide model tools for the development of anti-chronic hyperuricemia nephropathy drugs.

　　Methods: 40 male SD rats were randomly divided into 5 groups: normal group, group A (2? potassium acid + 12? mother paste +86? regular diet), group B (0.15? purine) + 10? mother paste + 89.85 Regular diet), group C (100 mg/kg adenine + 1500 mg/kg potassium oxazine, once a day in the morning and evening) and group D (50 mg/kg adenine + 1500 mg/kg potassium oxazine, single gavage Take medicine in the morning). The observation period was 5 weeks, and the weight of each group of rats, rat serum uric acid, creatinine, urea nitrogen and other indicators were measured every week. After 5 weeks, the ratio of rat kidney weight to body weight was as follows: the kidney was measured and observed through pathological sections.

　　Result: Compared with the normal group, the rats in group C lost weight, the ratio of kidney weight to body weight increased, blood uric acid, creatinine, and urea nitrogen increased, and the kidney color changed significantly. HE and urate staining shows the kidneys.

　　Conclusion: 100 mg/kg adenine + 1500 mg/kg potassium oxalate, daily morning and evening forced feeding to establish a rat model of chronic hyperuricemia nephropathy has the best effect.