动物造模,糖尿病 z-bio 2021-07-29 468

　　OBJECTIVE: To detect the expression levels of bladder smooth muscle contraction markers and the main regulatory gene myocardin in diabetic SD rats 9 weeks after the establishment of streptozotocin, and to understand whether the bladder smooth muscle of diabetic rats has phenotypic changes.

　　Method: 32 8-week-old male SD rats, weighing 200-220g, were randomly divided into diabetic (DM) group and non-diabetic (NDM) group. Nine weeks later, the bladder tissue was collected and observed for HE and Masson's trichrome staining. Changes in bladder tissue, qRT-PCR and Western blotting were used to detect bladder tissue smooth muscle actin (α-SMA), smooth muscle myosin heavy chain contraction smooth muscle markers, and myocardin gene mRNA and protein expression levels.

　　Results: Compared with the NDM group, rats in the DM group were significantly thinner (286.25±71.20 g vs 412.71±102.74 g, P = 0.001), polydipsia, polyuria, and increased collagen fibrous tissue in the bladder (P\u003c0.001) ), myocardin, α-SMA and smooth muscle myosin heavy chain mRNA and protein levels were significantly reduced (both Pu003c0.05).

　　Conclusion: The bladder smooth muscle of diabetic rats has phenotypic changes after 9 weeks of modeling, relaxing and contracting the bladder smooth muscle. It can play an important role in the pathological changes of diabetic bladder.