动物造模,流感病毒气溶胶感染模型 z-bio 2021-07-29 381

　　Objective: To infect three strains of mice to produce aerosols through aerosol challenge, analyze the characteristics of the three strains of mouse influenza model, and compare the infection differences of the three strains of mice during the infection process. Research the etiology of influenza and develop vaccines and drugs to select suitable infection models.

　　Method: A/PutoRico/8/34 (H1N1) virus strain and aerosol challenge method were used to weigh the three inbred C57BL/6, BALB/c and non-inbred ICR strains every day. The body weight of the mice was observed by naked eyes, and the mice were sacrificed on the 3rd, 7th, and 14th days after infection, and the lungs were taken to weigh the wet weight, and the lung virus was measured and pathological observation was performed.

　　Result: All three strains of mice can be infected. Among them, the survival rate of C57BL/6 mice is lower than that of the other two strains. The lung index and viral load of 3d mice were significantly higher than ICR. Mouse (Pu003c0.05). The pathological results under the microscope are more obvious than the other two strains. Compared with the other two strains of mice, BALB/c mice have the slowest weight recovery in the later stage, and the survival rate is higher than C57BL/6 but lower than ICR. Lung index and viral load are the other two strains. Importance: The pathological changes under the microscope are similar, but weaker than C57BL/6 and stronger than ICR. The pathogenesis of ICR mice is basically similar to that of the other two strains, but the body weight, survival rate, lung index, viral load, and pathological changes under the microscope are weaker than the other two strains.

　　Conclusion: All three strains of mice can establish influenza aerosol models, but the three post-infection models have their own characteristics. The experiment allows you to choose the right mouse strains and build models for various research goals.