动物造模,可诱导性肝损伤模型 z-bio 2021-07-29 442

　　OBJECTIVE: To establish an Alb-cre/DTR double transgenic mouse model, analyze related phenotypes, and establish an induced liver injury model on this basis for research on liver diseases.

　　Method: After breeding the introduced Alb-cre mice and DTR mice, mate them to obtain double transgenic mice. DNA was extracted from mouse tail tissue and genotyped by PCR. Double transgenic mice were intraperitoneally injected with diphtheria toxin, and blood was weighed at different time points to detect serum ALT and AST levels.

　　Result: Alb-cre and DTR mice were mated and screened to obtain Alb-cre/DTR double transgenic mice. The use of 0.625g/g of diphtheria toxin in mice can significantly increase ALT and AST in mouse serum. After the mice were dissected, the liver was observed to turn white. The results of HE staining showed obvious necrosis of liver cells.

　　Conclusion: We have successfully established a mouse model that may induce specific liver damage.