动物造模,大脑中动脉栓塞脑梗死模型 z-bio 2021-08-02 368

　　Objective: To explore the feasibility of using laser Doppler blood flow monitoring technology to establish a stable model of middle cerebral artery occlusive cerebral infarction (middle cerebral artery occlusion, MCAO) in rats.

　　Method: 16 SD male rats were randomly divided into 2 groups: 8 experimental groups and 8 control groups. The experimental group judged that the cerebral blood flow dropped to the basic value of 30? The model was successfully produced during the model making process. The control group did not monitor the cerebral blood flow and used a nylon tether with a depth of 1.8cm. I inserted it. I decided to create a successful model. The nerve injury severity score (mNSS) was performed before the model and 24 hours after the model. Rats were sacrificed 24 hours after modeling, and the brains were taken and stained with 2,3,5-triphenyltetrazolium chloride (TTC) to determine the cerebral infarct volume.

　　Result: 8 rats in the experimental group all showed typical symptoms of hemiplegia 24 hours after modeling. The mNSS score is stable at 10-13 points, with good infarct volume stability and uniformity, (37.5±3.9)? .. The mNSS scores of the eight rats in the control group are not stable. The mNSS scores of five rats ranged from 10 to 13 points. The cerebral infarction of 5 rats was similar to that of the experimental group, but 3 rats had cerebral infarction. It is significantly smaller than the experimental group (Pu003c0.05). The model-building success rate of the experimental group was 100? The model-building success rate of the control group was 62.5?Pu003c0.05).

　　Conclusion: Laser Doppler blood flow monitoring technology can significantly improve the success rate, stability and uniformity of the rat MCAO model.