动物造模,低钾型周期性麻痹模型 z-bio 2021-12-30 603

　　Objective: To establish and evaluate a hypokalemic periodic paralysis model by using gene knock-in CaV1.1-R528H mice.

　　Methods: 8-week-old gene knock-in CaV1.1-R528H male mice and 36 8-week-old wild-type C57BL/6J male mice, using 3 factor 2 level 2×2×2 factor planning method based on body weight, according to The principle of randomization is divided into eight groups (three factors of mutation, thyroxine and insulin, with or without two levels). Among them, mice in the thyroxine treatment group were intraperitoneally injected with levothyroxine sodium and weighed 350 μg/kg for 12 days to prepare for hyperthyroidism. After the last administration, the insulin treatment group was given a short-acting intraperitoneal injection. Detect and record each group of mice before injection (0 min) and after injection (30, 60 min) 0.8 U/kg body weight insulin and serum potassium.

　　Results: (1) The mice prepared with hyperthyroidism showed hypersensitivity, hypersensitivity, and dry fur. Compared with the control group, the food intake and water intake increased significantly, and the weight gain increased. It was too late. Thyroid function examination showed that T3 and T4 were significantly higher than the corresponding control, and TSH was significantly lower than the corresponding control, indicating that the difference was significant (Pu003c0.05). (2) When tyrosine or insulin were treated alone, there was no significant difference in serum potassium between the mutant group and the wild group at the same time point. After high tyrosine insulin treatment, the mutant group and the wild group were both at the same time point (30. , 60 minutes), the serum potassium of the mutant group was significantly lower than that of the wild group (Pu003c0.05). (3) Main effects and interactions: mutation alone or thyroxine factor does not affect serum potassium, only insulin affects the decrease of serum potassium (Pu003c0.05); sudden interaction between mutation factor and insulin factor with thyroxine factor (Pu003c0.05) ). 05); There is no interaction between thyroxine factor and insulin factor.

　　Conclusion: (1) Preparation for hyperthyroidism is successful. (2) Gene knock-in CaV1.1-R528H mice successfully established hypokalemia hypokalemia periodic paralysis model.