动物造模,中重度脓毒症长期生存大鼠模型 z-bio 2021-08-02 664

　　Objective: To establish a long-term survival rat model of moderate to severe sepsis, observe the natural progression of sepsis, and provide new ideas and methods for the study of sepsis.

　　Method: 40 SD rats were divided into a control group (sham group, 8) and a cecal puncture group (cecal puncture group, CLP group, 32). All operations were performed under the small animal anesthesia machine and sevoflurane inhalation. anaesthetization. All rats have established arteriovenous access to the right neck. After recovery 24 hours after surgery, rats in the CLP group developed sepsis due to appendicitis ligation and perforation. After the operation, the rats were moved to the recovery room (room temperature 22-25°C) and kept in a cage. On the 1st and 2nd day after operation, intravenous infusion of 6?AS and 5?glucose at a ratio of 1:1 to 20mL/kg/12h was carried out, after which the rats started to eat. According to the survival rate of rats, rats in the CLP group are naturally divided into survival group and death group. The rats were observed for up to 30 days after the operation, symptoms, weight changes, and plasma IL-10 levels were recorded, and changes in abdominal organs were observed through anatomical structures.

　　Results: (1) The survival rate within 24 hours of the CLP group was 75? The survival rate within 72 hours was 62.5? The survival rate within 7 days was 50? (2) According to the sepsis severity assessment system, 32 rats in the CLP group were postoperative Achieve moderate to severe sepsis within 24 hours. (3) Both the survival group and the sham group lost weight. The number of rats in the survival group decreased significantly from day 4 (P = 0.017). The surviving group had the lowest weight at 6 days postoperatively, and was lower than the original weight (8.51±2.23). The sham operation group had the lowest weight at 4 days postoperatively (2.73). The difference between the groups is ±1.82)? It is statistically significant in the original body weight (P=0.026). Thirty days after surgery, the two groups of rats (sham group (16.16±2.39)? survival group (13.03±3.74)? There was no significant difference in maximum weight gain (P=0.29). 4) Compared with the preoperative (0d) day , The plasma IL-10 concentration of the three groups all increased, the survival group (P=0.000) and the death group (P=0.010) both increased significantly. ...See Abdominal Anatomy: Dead group A lot of smelly ascites, the ligated intestinal tube is purple-black, not bandaged, and no adhesions. It ligates the intestines, but does not envelop ascites. There was no abnormality in the anatomy of the sham group. There was no abnormality in the surviving group. The spleen of rats accounted for (2.64±0.37)‰ of the body weight, and the spleen of rats in the sham group accounted for (1.63±0.20)‰. The difference between the two was statistically significant (P = 0.032).

　　Conclusion: This experiment is to establish a CLP model for long-term survivors of moderate to severe ascites. CLP and the model basically follow the onset of ascites and control how to screen.