动物造模,自发与诱发2型糖尿病 z-bio 2021-08-02 735

　　Objective: To compare the differences in glucose metabolism, insulin resistance and inflammation between spontaneous and induced type 2 diabetes mouse models.

　　Method: Animals are divided into normal control group, challenge model group, and spontaneous model group, with 10 animals in each group. Normal control group: normal 11-week-old C57BL/6J mice; induction model group: use high-fat diet combined with STZ to induce 11-week-old C57BL/6J mice to establish a diabetes model. Spontaneous model group: select 8-week-old C57BL/KsJ-db/ db mice; monitor changes in body weight, fasting blood glucose, glucose tolerance, FINS, AUC, HOMA-IR, and detect changes in TNF-α within 8 weeks. The contents of IL-18, IL-1β, and INF-γ before and after the experiment; 8 weeks later, the animal’s liver, kidney, pancreas, testis, skeletal muscle and other tissues were sacrificed for pathological analysis and collection.

　　Result: During the experiment, the weight of the spontaneous model group continued to increase, which induced a significant decrease in the weight of the model group. After 8 weeks, the blood glucose level of the model group was induced to decrease, while the blood glucose level of the spontaneous model group remained unchanged. The hyperglycemia of the spontaneous model group was stable, the FINS of the induced model increased, and the AUC and HOMA-IR were significantly reduced. There was no significant change in the FINS of the spontaneous model group. Both AUC and HOMA-IR increased significantly. 18. IL-1β, INF-γ) content is higher than the normal control group. At the 8th week, the pro-inflammatory factor content of the stimulating model group decreased, and the pro-inflammatory factor content of the spontaneous model group was significantly increased. The pancreas and other tissues of the two model mice had obvious inflammatory lesions. ..

　　Conclusion: After 8 weeks, the abnormal glucose metabolism and inflammation in the spontaneous type 2 diabetes model gradually increased, and the symptoms of hyperglycemia were maintained for a long time. The glucose metabolism and inflammation in the induced type 2 diabetes model were partially relieved after 8 weeks of growth. Partial improvement in blood sugar symptoms.