动物造模 z-bio 2021-08-02 947

　　Objective: To establish an animal model of body damage caused by chronic inhalation of atmospheric fine particulate matter (PM2.5) aerosol.

　　Method: Prepare PM2.5 aerosol continuous inhalation device. Investigate different concentrations (100±10 μg/m3, 150±10 μg/m3, 200±10 μg/m3), different times (1 week, 2 weeks, 4 weeks, 8 weeks, 12 weeks), and different methods (not Exposure to the effects of intratracheal perfusion and aerosol inhalation) and different animals (Wistar rats, BN rats and guinea pigs) on the establishment of the model. Observe the general state of the animals and calculate the weight growth rate and mortality; test lung function including respiratory rate, forced expiratory volume, occupancy vital capacity ratio (FEV1/FVC) and arterial oxygen partial pressure (PO2); count bronchoalveolar lavage fluid (BALF) ) Cell classification; light microscopy to check trachea and lung tissue pathology. Taking pneumonia in pathology of trachea and lung tissues as the criterion for establishing a successful model, determine the influencing factors of establishing the model.

　　Results: After continuous inhalation of PM2.5 aerosols of different concentrations for 8 weeks, Wistar rats became poisoned, weight gain was slow, the total number of white blood cells and mononuclear cell counts in the bronchoalveolar lavage fluid (BALF) increased, the ratio of macrophages increased, and the lungs Among the functional indicators, respiratory frequency increased, arterial blood PO2 and FEV1/FVC decreased, pathology showed inflammation and pulmonary fibrosis changes in trachea and lung tissues. Inflammation changes were positively correlated with exposure concentrations, but high concentrations had high mortality. Inhalation of the same concentration for different periods of time (1 week, 2 weeks, 4 weeks, 8 weeks and 12 weeks), the pathological changes of the trachea and lung tissues of the Wistar rats infected animals were significant from 8 weeks but no difference from 12 weeks. Different methods (non-exposure intratracheal perfusion method and aerosol inhalation method) Wistar rats were continuously inhaled for 8 weeks, and the two methods had similar effects, but the inhalation method had low animal mortality. Wistar rats, BN rats and guinea pigs were inhaled continuously for 8 weeks. The effects of the three animals were equivalent but the mortality rate of Wistar rats was low.

　　Conclusion: Using Wistar rats to inhale PM2.5 aerosol with a concentration of 150±10 μg/m3 for 8 consecutive weeks can establish a suitable animal model of PM2.5-induced body damage, and study and prepare a similar pathogenicity to PM2.5 damage to the body Environment, take the lead in establishing a model that is not only suitable for a large scale in the country, but also has the characteristics of Fujian Province.