动物造模,哮喘模型 z-bio 2021-08-02 406

　　Objective: To observe the effect of paeoniflorin on airway inflammation chemokines and receptors in asthmatic mice.

　　Method: Use OvalBmin (OVA) to sensitize and challenge the establishment of a mouse asthma model. Serum IL-6 and TNF-α levels and bronchoalveolar lavage fluid (BALF) ovalbumin-specific IgE (OVA)-ELISA method to detect IgE) and chemokines CCL19 and CCL21 levels; detection of lung tissue chemokine expression Westernblot, It is the recognized RT-PCR method to detect the expression of CCR7 and nuclear factor-κB (NF-κB) protein in lung tissue.

　　Results: The levels of serum IL-6 and TNF-α in the paeoniflorin intervention group were significantly reduced; the levels of BALFOVA-IgE, CCL19, and CCL21 were significantly reduced; the levels of CCR7 mRNA, CCR7 and NF-κB in lung tissue were significantly reduced. .

　　Conclusion: Paeoniflorin has a significant inhibitory effect on airway inflammation chemokine CCL19/CCL21 and its receptor CCR7 in asthma model mice.