动物模型 z-bo 2020-08-08 713

　　(1) Reproduction method Take type 3 capsular Streptococcus pneumoniae. The bacteria are inoculated twice to increase their virulence after live inoculation of mice. The bacteria solution is adjusted to a concentration of 1.2×1000000000CPU/ml with sterile normal saline. Male KM mice were anesthetized by intramuscular injection of ketamine at a dose of 150 mg/kg body weight. 4 drops of bacterial solution were instilled into each nostril of each mouse.

　　(2) Model characteristics Mice can show symptoms of acute sinusitis after being inoculated with bacteria. On the second day of modeling, hemorrhagic inflammation can occur in the nasal cavity and sinuses of the model mice; on the 8th day, the nasal cavity and sinuses of the animals can appear Neutrophils infiltrate; inflammation can reach a peak on the 11th day, and the inflammatory response gradually decreases on the 21st day, and the inflammation basically disappears until 28 days after the inoculation and infection.

　　(3) Comparative Medicine Rhinosinusitis is one of the common and frequently-occurring diseases in clinical otorhinolaryngology. Severe patients can cause olfactory disorders and affect the quality of human life. The main pathogens of sinusitis are Streptococcus pneumoniae (40%), Staphylococcus, Haemophilus influenzae and other aerobic and anaerobes. At present, the related fields have explored and established various animal models of nasal cavity and sinusitis such as rabbits, rats and mice, and have used these models to conduct in-depth research on nasal cavity and sinusitis. Rabbits were once considered to be the best animal model of rhinosinusitis. However, in recent years, there has been an increasing number of studies using rats and mice to make acute and chronic sinusitis models at home and abroad. Streptococci used for modeling infections are mostly more pathogenic. Strong Streptococcus pneumoniae type 3. This model does not require surgery, the preparation method is simple, the model features appear quickly, and the symptoms of sinusitis can be replicated in a short time. However, due to the differences in the anatomical structure of the nasal cavity and sinuses of mice and humans, this model is more suitable for nasal cavity and sinuses. Experimental research on screening of inflammatory drugs.