CRISPR/Cas9系统 z-bio 2021-08-03 239

　　Professor Yuan Jing's group from the School of Life Sciences, Xiamen University used the CRISPR/Cas9 system to efficiently implement different types of modifications of multiple genes in the Plasmodium genome, including gene deletion, gene tagging, and allele replacement.

　　Malaria is an infectious disease caused by the infection of Plasmodium parasites. It is still a serious public health burden on a global scale. Although the Plasmodium genome has been sequenced, the function of most genes in its genome is unknown. The original methods of editing and modifying the Plasmodium genome are extremely inefficient, which greatly limits the discovery of the function of the Plasmodium gene.

　　In this study, Professor Yuan Jing’s group from the School of Life Sciences, Xiamen University introduced a site-specific DNA double-strand break into the Plasmodium yoelii genome based on a new genome modification method CRISPR/Cas9 system. Source recombination repair can efficiently realize different types of modification of multiple genes in the Plasmodium genome, including gene deletion, gene tagging and allele replacement. The development of this system has improved the ability of researchers to modify the Plasmodium genome, thereby facilitating the study of Plasmodium biology.