动物造模,动物实验 z-bio 2021-08-04 284

　　Objective: To observe the effects of Tanreqing injection on the cardiovascular system, beagle respiratory system and central nervous system of ICR mice, and to provide experimental data for the safety of clinical administration.

　　Methods: 24 Beagle dogs were divided into two halves for men and women, random low-dose (2.5mL stock solution/kg), medium-dose (5.0mL stock solution/kg), high-dose (10.0mL stock solution/kg) subcutaneously intravenously under anesthesia and bolus injection on the right forefoot , Tank injection group, solvent control group, 6 animals per group according to body weight, before administration, 40 minutes after administration, 80 minutes after administration, 5, after administration, 10, 20, 30 and 30. Blood pressure, heart rate, electrocardiogram, and breathing at 60, 90, 120, 150, 180, 210, and 240 minutes. There were 160 ICR mice, half males, 60 of which were randomly low-dose (3.6 mL stock solution/kg), medium-dose (6.25 mL stock solution/kg) and high-dose (12.5 mL). Stock solution/kg). ) Depends on your weight. In the injection group, solvent control group and positive control group, the general behavior and white hair activity of the mice were observed after tail vein injection; 50 mice in the same group were measured at different time points after administration; the others were 50 mice. In the same group, the synergistic effect of sodium pentobarbital after administration of subthreshold sleep dose was determined.

　　Results: There was no significant difference in systolic blood pressure, mean arterial pressure, electrocardiogram or respiratory frequency between the Tanreqing injection dosage group and the solvent control group at the same time point before self-administration (P\→ 0.05). ), at each time point The difference between diastolic blood pressure and heart rate was statistically significant (Pu003c0.05 or Pu003c0.01), but no significant dose or time effect correlation was found. After administration, the spontaneous activity of ICR mice in the high-dose group decreased at each time point (Pu003c0.05, Pu003c0.01 or Pu003c0.001), and the coordination evaluation was 30, 50, and 90. Significantly increased. A few minutes after administration (Pu003c0.05, Pu003c0.01 or Pu003c0.001). 0.05, Pu003c0.01 or Pu003c0.001).

　　Conclusion: Under the experimental conditions, a single intravenous injection of Tanreqing injection has no significant effect on the cardiovascular and respiratory systems of Beagle dogs. Large-dose Tanreqing injection has a certain effect on the central nervous system of ICR mice. It manifests as decreased spontaneous activity and coordination disorder, which may be related to its main active ingredient, bear bile powder.