动物造模,急性心肌梗死 z-bio 2021-08-04 227

　　Objective: To investigate the effect of cord blood mononuclear cells (HCMNC) on angiogenesis in rats with acute myocardial infarction (AMI) and its mechanism.

　　Method: I separated HCMNC and labeled it with BrdU. Select 25 rats to establish a rat model of acute myocardial infarction by coronary artery ligation. The 20 successful models were randomly divided into 10 groups and 10 groups of myocardial infarction group and HCMNC group. mock surgical group. After the model was successfully established, the rats in the HCMNCs group were injected with HCMNCs around the myocardial infarction, and the sham operation group and the model group were injected with L-DMEM medium at the same site. Four weeks after transplantation, echocardiography was used to detect cardiac function, HE staining was used to observe myocardial pathological changes, and BrdU staining was used to detect the survival and immunity of transplanted cells. Microvessel density (MVD), CD31, and vascular endothelial growth use histochemical staining (VEGF) to detect factors at the edge of myocardial infarction. ) Protein expression, Western blotting was used to detect CD31 and VEGF protein levels in myocardial tissue.

　　Results: Compared with the sham operation group, left ventricular end systolic diameter (LVED) and left ventricular end diastolic diameter (LVEDd) levels increased in myocardial infarction group and HCMNC group, left ventricular ejection fraction (LVEF) left and shortened myocardial infarction Compared with the HCMNC group, the LVED and LVEDd levels of the ventricular short axis shortening in the HCMNC group decreased, and the LVEF and FS levels increased (Pu003c0.05). With HE staining, the structure of myocardial cells in the sham operation group was normal, and the myocardial cells in the myocardial infarction group were arranged randomly, with a large number of inflammatory cell infiltration. The structure of cardiomyocytes in HCMNC group was basically normal. .. BrdU staining showed that no BrdU-labeled positive cells were detected in the sham operation group and the myocardial infarction group; there were scattered BrdU-positive cells in the infarct area of the HCMNCs group, which participated in the formation of the blood vessel wall. Compared with the sham operation group, the CD31 and VEGF protein optical density values, relative protein expression and MVD of the myocardial infarction group and HCMNC group decreased (Pu003c0.05); compared with the myocardial infarction group, CD31, CD31 and MVD decreased. The density of VEGF protein and HCMNCs group, relative protein expression level and MVD increased (Pu003c0.05).

　　Conclusion: HCMNC can promote the establishment of collateral circulation in AMI rats, induce angiogenesis, and significantly improve ischemic heart function.