动物造模 z-bio 2021-08-04 215

　　Objective: To investigate the palliative effect of electroacupuncture on the anxiety behavior of pain aversion rats and its effect on the anterior cingulate gyrus (ACC) N-methyl-D-aspartic acid (NMDA) and γ-aminobutyric acid (GABA) receptors Impact.

　　Method: All healthy rats were completely randomly divided into blank (NS) group, model (C-CPA) group, conventional electroacupuncture (EA1) group, and pre-electroacupuncture (EA2) group. The Freund's adjuvant induction conditions were established. Complete Freund’s adjuvant-induced conditioned place avoidance (C-CPA) pain aversion rat model. The EA1 group was treated with electroacupuncture after modeling, and the EA2 group was treated with electroacupuncture 3 days before modeling. The mechanical foot withdrawal threshold (PWT) and anxiety behavior of rats were observed; Western Blotting detected GABA receptor GABAAR and GABA in ACC. GABABR, NMDA receptor R2A, NR2B protein content.

　　Results: Compared with the NS group, the escape scores (CPAscores) of the C-CPA group, EA1 group and EA2 group were significantly reduced (P\u003c0.05). Compared with the S group, the GABAAR protein expression in the affected ACC in the C-CPA, EA1 and EA2 groups was significantly increased (P0.05), and the GABAAR protein expression in the EA2 group was significantly decreased (P0.05). .. There was no difference between the GABABR, NR2A and NR2B protein expression groups in the affected ACC (P\u003c0.05).

　　Conclusion: Electroacupuncture at different times can reduce the pain and anxiety behavior of C-CPA pain aversion model rats. The pre-electroacupuncture effect may be related to the inhibition of GABAAR protein expression in the affected ACC.