动物造模 z-bio 2023-03-10 582

　　Objective: To investigate the effect and mechanism of Shenqu Xiaoshi oral liquid on gastrointestinal motility in mice with functional dyspepsia (FD).

　　Method: 40KM mice were randomly divided into normal group and Shenqu Xiaoshi oral liquid low, medium and high dose groups, and the blood routine and liver and kidney function indexes of the mice were tested. In addition, 50KM mice were randomly selected as the normal group, and the remaining mice were randomly divided into the model group, and the FD animal model was constructed by the irregular diet + L-arginine (L-Arg) method. Shenqu Xiaoshi oral liquid low, medium and high dose groups; record mouse body weight, calculate mouse survival and intestinal progress, observe pathological changes of stomach tissue, real-time fluorescent quantitative PCR (qRT-Use PCR) and Western blot. (Western blot) To detect the expression of endoplasmic reticulum stress factor inositol-requiring enzyme 1 (IRE1) and tumor necrosis factor receptor-related factor 2 (TRAF2) in mouse gastric tissue.

　　Results: Comparison of amino acid transfer in mouse white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), platelets (PLT), lymphocytes (LYM), monospheres (MONO), neutrophils (NEU), and asparagus groups Enzyme (AST), alanine aminotransferase (ALT), total bile acid (TBA), urea nitrogen (BUN), creatinine (CRE), no significant difference between the groups (P\u003c0.05) Mucosal layer The gastric and serous submucosa and muscles of the mice in each group have clear structures without obvious pathological changes. Compared with the model group, Shenqu Xiaoshi oral liquid has significantly increased gastric emptying and small intestinal progression rates in the low, medium and high dose groups. The expression of TRAF2 mRNA and protein in tissues was significantly reduced (Pu003c0.05).

　　Conclusion: Shenqu Xiaoshi oral liquid has no obvious effect on blood routine and liver and kidney function of normal mice. It can greatly improve the gastrointestinal motility of FD mice.