动物造模 z-bio 2021-08-04 587

　　OBJECTIVE: This article uses leptine receptor (LEPR) gene knockout rats to analyze the changes in the morphology and function of microglia in the rat brain after the leptine receptor gene is knocked out, and to explore the role of leptin receptor The role of cytoplasmic effect.

　　Method: Use T-PCR, Western blot, immunohistochemistry and immunofluorescence to observe the activation of rat leptin receptor in vivo and in vitro.

　　Result: Leptin receptor is expressed in microglia, gene knockout can completely eliminate LEPR protein in microglia. LEPR knockout can enhance the inflammatory response of rat LPS to stimulation and reduce the survival rate by 75? In addition to activated microglia, LEPR knockout also significantly increases the proportion of activated microglia in the rat brain . LEPR knockout primary microglia not only secrete more inflammatory factors, but also increase their phagocytic ability. Western blot showed that PI3K/AKT knocked down the leptin receptor. The protein in the rat brain tissue was significantly enhanced.

　　Conclusion: After knocking out the leptin receptor, rat microglia develop to promote inflammation and phagocytosis, revealing that LEPR/leptin may regulate neuroinflammation through microglia.