动物造模,人脐带间充质干细胞 z-bio 2023-03-23 455

　　Purpose: In this study, human umbilical cord mesenchymal stem cells (hUCMSC) transplantation can improve the decline of learning, memory and cognitive ability in naturally aging rats by regulating the level of autophagy. To study whether it is feasible.

　　Methods: SD rats were reared in a barrier environment for anti-aging (24 months of age). In the behavioral study, aged rats with significantly reduced cognitive ability were selected. Cell transplantation group (H group) and saline group were divided into (C group) ). ), 3-month-old SD rats are the normal group (N group). Group H; 500 μL hUCMSC cell suspension was injected into the tail vein, the dose was 2×10*. Both groups C and N were injected with sterile saline through the tail vein. The time is once a week for 4 consecutive weeks. The Morris water maze, Y maze, and changes in learning and memory abilities were observed through new object recognition experiments, and the relative expressions of rat hippocampal neurons, hippocampal LC3II/I, Beclin1 Westem Blot and P62 were observed by HE staining.

　　Results: (1) Behavioral results showed that the learning and memory ability of group H and group N was significantly higher than that of group C. (2) HE staining, the nerve cells in the CA1 and DG areas of the hippocampus in the H and N groups are arranged regularly, the cell structure is complete, and the nucleus is full. Group C decreased, the cells were loosely arranged, the nuclei contracted, and degenerative lesions appeared. (3) The P62 protein content of group H and group N was significantly lower than that of group C, and Beclin1 and LC3II/I were significantly higher than that of group C.

　　Conclusion: Intravenous injection of human umbilical cord mesenchymal stem cells can improve the decline of learning, memory and cognitive ability in naturally aging rats.