动物造模,无菌大鼠培育 z-bio 2021-08-05 273

　　Purpose: In the cultivation of sterile rats, artificially prepared milk after birth is very important for the cultivation of sterile rats. Deeply hydrolyzed protein lactose-free milk powder is easily absorbed by babies, reducing various indigestion and allergic reactions. Secondly, adding this milk powder to the milk used by sterile rats has a positive effect on the cultivation of sterile rats. Detect the biochemical indicators of sterile rats fed normally.

　　Method: Use clean SD mice to determine working hours. After excision of the uterus, the pups were peeled off with an isolator and manually fed until 22 days after weaning to record body weight and survival rate. .. In the milk powder used, the experimental group added milk powder without highly hydrolyzed protein lactose, and the control group added regular milk powder. Normally cultured sterile rats were tested for blood biochemical indicators at the 8th week.

　　Result: In the first 14 days of artificial feeding, there was no difference in body weight and survival rate between the experimental group and the control group. From the 14th day, the body weight and survival rate of the experimental group began to increase. Until the 22nd day, the survival rate of the control group and the experimental group increased significantly (37.18 17.78?, and was significantly higher than the control group (9.96 ± 0.49) on the 22nd day. Yes (13.36 ± 0.59). Sterile rats Blood biochemical indicators showed that AST (aspartate aminotransferase) decreased, and GLU (glucose) increased.

　　Conclusion: In the process of raising sterile pups, lactose-free milk powder containing deeply hydrolyzed protein can be added as an important ingredient of formula milk. This can effectively increase the weight of young rats and reduce their mortality. The blood biochemical indexes of female infertile rats changed significantly.