动物造模,心脏肥大大鼠 z-bio 2021-08-05 656

　　Objective: To search for important biomarkers through the serum metabolome and to explore the etiology of myocardial hypertrophy in rats.

　　Method: Inject 30 mg/(kg?d) of isoproterenol intraperitoneally for 14 consecutive days to establish a rat model of myocardial hypertrophy. The heart mass index is used to evaluate the rat model of cardiac hypertrophy. Ultra Performance Liquid Chromatography-Use quadrupole time-of-flight tandem mass spectrometry to detect endogenous metabolites in rat serum, use MPP software to analyze metabolite differences, Human Metabolome Database (HMDB) This database is used to determine organisms landmark. Used to analyze metabolic pathways.

　　Result: Intraperitoneal injection of isoproterenol can induce myocardial hypertrophy in rats. There are significant differences in serum metabolites between the myocardial hypertrophy model group and the normal group, and a total of 10 potential biomarkers have been identified. Compared with the normal group, the myocardial hypertrophy model group's sphingosine 1-phosphate and dihomo-γ-linolenic acid were significantly down-regulated, D-1,6-diphosphate glucose, deoxyadenosine, and N-acetylmethionine Acid, phytosphingosine, allantoin, 3-keto-β-D-galactose, octane and glycerol were significantly up-regulated.

　　Conclusion: Myocardial hypertrophy induced by ispinalin includes metabolic pathways such as sphingolipid metabolism, glycerolipid metabolism, galactose metabolism, unsaturated fatty acid biosynthesis, purine metabolism and so on. This study provides a reference for revealing the metabolic changes of circulating blood in myocardial hypertrophy induced by isoproterenol.