动物造模 z-bio 2021-08-05 334

　　Objective: To study the influence of western diet and standard diet on hematology and pathology of apolipoprotein E knockout mice (ApoE-/-).

　　Method: Western food and standard diet were fed to 3-month-old ApoE-/- mice, and the peripheral blood and aortic arch of the mice were collected at 3, 4, and 5 months of feeding. An automatic blood analyzer detects blood lipid levels, and slices are stained to observe the formation of atherosclerotic plaques.

　　Results: Regarding blood lipids, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in serum of mice fed with Western diet and standard diet. C57 mice were raised 4 Except for HDL-C, the blood lipid index of the western food group was significantly higher than that of the standard diet group. The aortic arch staining and western-style food showed obvious aortic plaques, and the incidence of AS was significantly increased.

　　Conclusion: Western and standard diet can induce ApoE-/- mice to show AS symptoms, and Western diet can cause more obvious effects.