动物造模 z-bio 2021-08-05 377

　　Objective: To inject cyclophosphamide intravenously into pregnant rabbits, observe the toxic effects and performance of pregnant rabbits on embryos and feet, and provide a reference for selecting cyclophosphamide as a positive control.

　　Method: The pregnant rabbits were randomly divided into a control group [0.9? sodium injection, GD11 (GD, pregnancy day), iv] and a cyclophosphamide group (cyclophosphamide 30mg/kg body weight, GD11, iv). I did it. GD28 euthanized a pregnant rabbit, collected the fetus by Caesarean section, and examined the appearance, internal organs and bones of the fetus.

　　Result: The cyclophosphamide group has no obvious effect on the rabbit's reproductive function and embryogenesis, and can cause the appearance, internal organs and bone deformation. The occurrence rate of cleft palate fetuses is 32.95? Visceral changes are 10.23? Bone deformities are 23.37?

　　Conclusion: In New Zealand, intravenous injection of cyclophosphamide 30 mg kg-1 without GD11 can cause fetal malformations. The type and incidence of deformity are appropriate and can be used as a positive control for reproductive tests.