动物造模,重度创伤失血性休克模型 z-bio 2021-09-26 460

　　Objective: To establish an improved rat model of severe traumatic hemorrhagic shock, and to explore the protective effect of anesthesia on the establishment of STHS rat model.

　　Method: Select 40 adult healthy male SD rats and randomly divide them into 4 groups. The normal group was anesthetized with bilateral femoral artery and left femoral vein catheters, waiting for the rats to wake up or maintain anesthesia, and then divided into the awakening control group. (CC, n=10), the anesthesia control group (AC, n=10), and the traumatic shock group were all used open wound combined with anesthesia or regular quantitative control of bleeding under awake conditions. Each rat model caused severe traumatic hemorrhage. shock. Traumatic hemorrhagic shock group (CTHS, n=10), anesthetic traumatic hemorrhagic shock group (ATHS, n=10). During the experiment, observe the general physiological state of each group of rats, and continuously monitor the hemodynamic indicators of experimental rats: mean arterial pressure (MAP), heart rate (HR), left ventricular pressure, maximum increase and decrease rate (±dp) /dtmax)), arterial blood gas index: pH, oxygen partial pressure (PaO2), carbon dioxide partial pressure (PaCO2), hemoglobin measurement (ctHb), lactic acid (Lac), blood sugar (Glu)), concentration of dioxide (cHCO3- ), Alkaline residue (BE); at the end of the experiment, observe the damage of the rat's heart, liver, lung, and kidney organs under a HE staining microscope.

　　Results: In the CTHS and ATHS groups, there were significant differences in HR and ±dp/dtmax over time (P\u003c0.01). The HR and ±dp/dtmax of the ATHS group showed a downward trend, while the CTHS group showed a downward trend. High or stable (T2, T3, T4, T5, T6) state first decreased (T1) and then increased; MAP showed a gradual upward trend after the shock in the ATHS group, and finally 25 0 has returned to the normal level? On the other hand, the CTHS group is in After compensating for the growth period, it drops rapidly. The PH, PaCO2, ctHb, cHCO3- and BE of CTHS group and ATHS group showed a gradual decrease. Except for PH and ctHb, the decline in CTHS group was significantly greater than that in ATHS group (Pu003c0.01); Glu, Lac, PaO2 showed a gradual upward trend, and CTHS group showed an upward trend. It was significantly higher than that in ATHS group (Pu003c0.01). Compared with the CTHS group, the liver, heart, lung and kidney damage in the ATHS group was significantly reduced.

　　Conclusion: Anesthesia helps to protect the establishment of severe traumatic hemorrhagic shock in rats, improve hemodynamics, reduce the accumulation of respiration and metabolites, and reduce the degree of damage to various organs.