动物造模,2型糖尿病 z-bio 2021-08-10 1484

　　Objective: To observe the changes of blood glucose levels in a mouse model of type 2 diabetes, and to explore the feasibility of using blood glucose levels 2 hours after a meal as a blood glucose intervention evaluation point.

　　Method: Choose healthy C57BL/6 mice, adopt high-fat diet and streptozotocin (STZ) to induce type 2 diabetes (T2DM) experimental group, set up normal control. Measure the continuous fasting and continuous random blood glucose of the two groups respectively, analyze the blood glucose law of the mice, and evaluate the scientificity of the blood glucose 2 hours postprandial as the evaluation point of blood glucose intervention in T2DM mice. Regularly observe the postprandial blood glucose fluctuations of diabetic mice 2 hours after the meal to confirm the postprandial blood glucose fluctuations. The stability of the end point of the 2-hour blood glucose intervention.

　　Results: The continuous fasting blood glucose levels of T2DM mice showed a relatively stable range of blood glucose levels during the 2-3 hour fasting period after a meal. After fasting for more than 4 hours, the blood glucose level of the T2DM group continued to drop. Significantly, the normal group can slowly increase and maintain, but the blood sugar is relatively stable; within 7 weeks, the blood glucose fluctuations of 2 hours after the meal of the T2DM mice showed that the blood sugar was 2 meals, and it was stable after a few hours.

　　Conclusion: The blood glucose level 2 hours after a meal can stably reflect the blood glucose change rule of the type 2 diabetes mouse model, and it has excellent application value in the evaluation of blood glucose intervention experiments.