动物造模,免疫力低下小鼠动物模型 z-bio 2021-08-10 768

　　Objective: To quickly establish a low-immune KM mouse animal model, and to determine the establishment of the animal model in a simple and rapid way.

　　Method: Gastrointestinal feeding of cyclophosphamide, 100 mg/kg, hydrocortisone feeding via stomach and intestine, 80 mg/kg, 60? Alcohol solution fed through stomach and intestine, 20 ml/kg, UVA skin irradiation ( Long wave)), the dose is 10 J/cm2, the weight of the mice before and after the test is measured, the content of IgG, IgA, and IgM in the mouse serum is measured, and the peripheral tissue of the mouse is measured by flow cytometry. .. Analyze the content of CD19 in blood B lymphocytes and CD3 in T lymphocytes with an automatic hematology analyzer.

　　Result: Since the hypoimmune model cyclophosphamide group and hydrocortisone group KM mice have been established, the hypoimmune model 60? alcohol group and ultraviolet irradiation group have not been established, the mice were weighed and measured. Further experiments are needed And investigate to detect it. IgG, IgA, IgM in serum, B and T lymphocytes in peripheral blood of mice, and routine analysis of mouse blood can be used as reference detection indicators to establish a weakened immune model of KM mice. ...

　　Conclusion: An animal model of hypoimmune KM mice was established by continuous intragastric administration of cyclophosphamide 3d 100mg/Kg. From the perspective of various testing indicators, body weight, serum immunoglobulin content, and the simplicity of blood routine, these three indicators are: easy to obtain, low testing cost, short duration, high testing efficiency, and can be used as daily testing Establish and confirm the indicators of animal models in the work.