动物造模,束缚应激模型 z-bio 2021-08-10 979

　　Purpose: Self-made mouse restraint device, established postpartum mouse restraint stress model, used for related stress experimental research.

　　Method: Choose a centrifuge tube of a size suitable for the size of the mouse, and prepare a simple mouse restraint device. On the second day after the mice gave birth, the device was used to fix and restrain the postpartum female mice on the device. This exercise is based on the separation of mother and child. Female rats are subjected to restraint stress for 3 hours every day for 21 consecutive days to prepare a restraint stress model for postpartum mice.

　　Result: In this postpartum mouse restraint stress model, the expression of ghrelin in the stomach, hypothalamus and serum increased, the expression of genes such as 5?HT2 receptors decreased, and symptoms such as depression were alleviated. Momentum and delayed growth of offspring.

　　Conclusion: This article introduces a restraint stress model in postpartum mice. Many experiments have shown that the modeling results are stable and reproducible, and can be used for experimental studies of restraint stress in mice.