动物造模,痔疮模型 z-bio 2022-02-01 576

　　Objective: To study the protective effect of hemorrhoid magnetization ointment on mice with hemorrhoids induced by acetic acid.

　　Method: 40 SPF ICR mice were divided into normal group (10) and model (30). This model uses 20? acid to establish a model of hemorrhoids. After 24 hours of successful model building, the model group, hemorrhoid ointment group, and Mayinglong musk hemorrhoid ointment group were treated accordingly. Perianal score was performed 11 days after treatment. The CBA kit was used to detect the levels of IL-6, IL-1β and TNF-α in the peripheral blood, and the Griess kit was used to detect the level of nitric oxide (NO) in the peripheral blood, and hematoxylin-eosin ( HE) staining to evaluate the pathological changes of rectal tissue.

　　Results: Compared with the normal group, the perianal score of the model group was significantly increased, the rectal tissue was seriously damaged, and the levels of IL-6, IL-1β, TNF-α, and NO in the peripheral blood increased significantly. The perianal score of each administration group was significantly reduced, and the pathological damage of rectal tissue was significantly improved. The levels of peripheral blood L-6, IL-1β, TNF-α, and NO were significantly improved compared with the model group, and the levels were significantly inhibited.

　　Conclusion: Hemorrhoid magnetization ointment has a good therapeutic effect on mouse hemorrhoid models, which is closely related to the regulation of inflammation in the body.