动物造模,动脉粥样硬化 z-bio 2021-08-13 590

　　Objective: To construct a rat model of atherosclerosis (qi stagnation and blood stasis syndrome) combining disease and syndrome, and to provide an evaluation tool for the pharmacodynamic research of corresponding Chinese herbal medicines.

　　Methods: Refer to relevant data and literature research, extract and evaluate the diagnosis and treatment indicators of atherosclerosis (qi stagnation and blood stasis syndrome) rat model, and use simvastatin tablets and Tongmai granules as counter-symptomatic drugs. Combined with the cause of atherosclerosis The evolution of pathogenesis and the modern research on pathogenesis, it is proposed to select the combined induction method of ice-water bath, gavage fat emulsion and tail vein injection of bovine serum protein to prepare atherosclerosis (qi stagnation and blood stasis syndrome) rat model of combined disease and syndrome. During the experiment, the symptoms and signs of the rats in each group were investigated and assigned points. Eight weeks after the model was made, the four levels of blood lipids and blood coagulation in each group of rats were tested, the pathological changes of the ascending aorta, and the model was pre-tested. After the model is successful, intervene with anti-symptomatic drugs, and measure the four levels of blood lipids and blood coagulation in rats, and detect the pathological changes of the ascending aorta and liver tissue.

　　Results: 21 weeks after modeling, compared with the normal group, the symptoms and signs of the rats in the model group changed significantly, the blood lipid level and fibrinogen content were very significantly increased, and the activated partial prothrombin time was significantly reduced. In the ascending aorta Lipid deposits under the membrane, vacuoles and degeneration of hepatocytes, which are basically in line with the clinical manifestations of atherosclerosis (qi stagnation and blood stasis syndrome). After drug intervention, compared with the model group, the blood lipid levels of rats in the drug contradiction group were The fibrinogen content was significantly decreased, and there were no obvious abnormalities in the ascending aorta intima and liver tissue.

　　Conclusion: The combined induction method of ice water bath, gavage fat emulsion and tail vein injection of bovine serum protein for 21 weeks can establish a rat model that simulates clinical atherosclerosis (qi stagnation and blood stasis syndrome), and has a model rate High, similar to clinical symptoms and signs, etc., which will provide evaluations for the pharmacodynamic research of anti-atherosclerosis (qi stagnation and blood stasis) Chinese herbal compound prescriptions