动物造模,人肝癌裸小鼠原位癌模型 z-bio 2021-09-17 749

　　Objective: How to select a human hepatocarcinoma cell line (HCCLM3-Luc) stably expressing the luciferase reporter gene to construct a nude mouse model of human hepatocarcinoma cell line carcinoma in situ?

　　Method: In vivo imaging of the HCCLM3-Luc fluorescence intensity expression of cells, used for quantitative analysis of different numbers, and detection of luciferase activity in each cell number, the linear relationship between the amount of photons produced by HCCLM3-Luc luciferase and tumor cells The number of relevance? The liver lobe was inoculated with HCCLM3- in situ, and the Luc CLM3-Luc cell suspension was injected into the tumor tissue and the tail vein, respectively, to construct a nude mouse intraepithelial model of human liver cancer. Compare the feasibility of the two methods, establish a nude mouse model of human liver cancer cell line, and establish a model?

　　Result: The number of HCCLM3-Luc cells is positively correlated (correlation coefficient 2 = 0.9989, linear equation: Y = 1155.8X + 1 × 106), and the average OI value is about 140 photons. Per unit/second? The in situ tumor formation rate in the liver lobe in situ immunization group [(82.5±7.2) was significantly higher than that in the tail vein injection group [(34.1±13.2)?

　　Conclusion: This project has constructed a bioluminescent human liver cancer nude mouse model of carcinoma in situ. Can it use a small animal imager to monitor tumor growth in real time?