动物造模 z-bio 2021-09-17 565

　　Objective: To establish a mouse model of intestinal disseminated endogenous infection, and provide a reliable experimental model for studying the association mechanism between intestinal flora and endogenous infection?

　　Method: 24 ICR female mice were randomly divided into model A group, model B group and control group C. The model A group was given a broad-spectrum antibiotic solution to destroy the normal intestinal flora. Have you been given 5-fluorouracil (5-FU) from the tail vein for immunosuppression? Is the model B group the basis for oral Candida albicans to introduce opportunistic pathogens into the model A group? Is it the same with normal saline for control group C? During the experiment, the changes in the fecal flora of the mice were continuously observed, and the mice were detected by the plate counting method. HE staining to observe the tissue load, pathological changes of mouse lung, liver, cecum and colon tissues, fluorescent quantitative PCR to observe the quantitative changes of the main intestinal microbiota of mice?

　　Result: At the end of the experiment, the bacterial infection of the tissues and organs of the model group A mice appeared in all the mice. Does the model group B mice have mixed bacterial and fungal infections? The lung and liver tissues and organs of the two model groups showed typical inflammatory reactions, and the mucosa of the caecum and large intestine had inflammation, which destroyed the integrity of the barrier. Quantitative results of intestinal flora showed that the structure of the main intestinal flora in the two model groups was disordered, and the resistance to intestinal colonization was reduced. The B/E value was u003c1?

　　Conclusion: The intestinal flora of mice is disordered and immunosuppressed. Next, the intestinal or opportunistic pathogens break through the intestinal mucosal barrier and cause tissue and organ infections. The basis of a reliable model for the prevention and control of endogenous infections from the perspective of intestinal microbiology?