动物造模 z-bio 2021-08-13 483

　　Purpose: Do you want to use BALB/c nude mice to establish an M1 leukemia model?

　　Method: 12 female BALB/cNude mice aged 5-6 weeks were randomly divided into low, medium and high dose groups and blank group. Only three groups of tail vein injections, low, medium and high, were inoculated with logarithmic growth phase M1 cell suspension at 1 x 106, 5 x 106 and 8 x 106. Observe the general condition of the mouse. Peripheral blood was collected at 0, 10, 20, and 30 days after modeling, and the white blood cell classification was tested in the blood routine. The samples were sacrificed on the 30th day or the day of death to determine the proportion of CD33 CD117 positive cells. Tissues were prepared by measuring the concentration in peripheral blood and bone marrow by flow cytometry. Pathology?

　　Result: The mice in the model group showed symptoms of malice, decreased activity, and arched spine after 10-15 days. Vaccination. On the 30th day of the experiment, the number of peripheral blood leukemia cells in the high-dose group was compared with the number in the blank group. It increased significantly (Pu003c0.05). The proportion of peripheral blood leukemia cells in each group was significantly higher than that of the blank group (Pu003c0.05); the ratio of CD33+CD117+ in the bone marrow of mice in each group increased, which was the most significant with the high-dose group. Yes (Pu003c0.05)); Is there leukemia cell infiltration in the spleen in the low-high-dose group?

　　Conclusion: BALB/cNude mice can be injected with 8×106 mouse leukemia cells M1 through the tail vein to construct an acute myeloid leukemia model. Does it conform to the biological characteristics of acute myeloid leukemia?