动物造模,耐药小鼠模型 z-bio 2021-09-19 347

　　Objective: To detect the effect of low-concentration levofloxacin on the pneumonia model of Pseudomonas aeruginosa (PA) infection in mice, and to explore the method of levofloxacin resistance?

　　Methods: Infect mice with PA to induce a pneumonia model. Each experiment was randomly divided into groups according to body weight, with different bacterial concentrations, number of infections, administration methods, changes in mouse body weight, and the end point as mouse lung indicators. The therapeutic effect of levofloxacin was analyzed, and Conduct a feasibility study. Results Low-concentration levofloxacin was an effective PA (1×109 cfu/mL) single infection in mice. The lung indicators of model 1 to model group 2 were significantly different from those of the normal group (P). 01). ); There is no significant difference between levofloxacin group 2 and model group 2. Compared with the normal group, low-concentration levofloxacin had a single infection in PA (1 x 1010 cfu/mL) mice. Model group 1 and model group 2 are significant. Poor (Pu003c0.01). Compare the levofloxacin group 3 with the model group 2. Are the results significantly different? Levofloxacin group 1-2 can significantly reduce the lung index of the model group. In the experiment of PA repeatedly infected mice, is there a significant difference between the model 1 group and the model 2 group compared with the normal group (Pu003c0.01)? Is there a significant difference between levofloxacin 2 group and model 2 group? In each experiment, the weight of the second mouse was lower than the normal group on the second day after infection. Is there a significant difference in the results?

　　Conclusion: Under the experimental conditions, the concentration of levofloxacin single infection PA mice is low (1×1010 cfu/mL)? Can the animal model levofloxacin in mice repeatedly infected with Pseudomonas aeruginosa at low concentrations simulate clinical drug resistance to a certain extent?