动物造模 z-bio 2021-08-13 253

　　Objective: How to establish a human prostate cancer xenograft model to evaluate the anti-tumor effects of various treatment options?

　　Method: Fresh human prostate cancer surgical specimens are mixed with Matrigel and transplanted into exogenous androgen supplement nudes. Tumor growth was evaluated for its fidelity and continuous passage; cancer-bearing mice were divided into four groups: docetaxel, castration, docetaxel combined castration and control, whether changes in tumor volume and body weight during treatment should be tested To evaluate the effect of treatment, serum total prostate specific antigen (tPSA) concentration and histopathological changes after treatment?

　　Result: We have successfully established a PDX model of prostate cancer, including hormone sensitivity (D17225). For castration-resistant (C40019) tumors, pathological analysis showed that the transplanted tumors better maintained the main characteristics of the patient's primary tumor. Does the latter D17225 model have a more obvious anti-tumor effect?

　　Conclusion: The PDX model of prostate cancer has been successfully established and stabilized. Docetaxel alone or in combination with castration has a significant therapeutic effect on hormone sensitivity (D17225) PDX model of prostate cancer?