动物造模,结核小鼠感染模型 z-bio 2021-08-13 445

　　Objective: To use two cultures of Mycobacterium tuberculosis to simultaneously verify the therapeutic effect of rifampicin (RIF) in a mouse model of pulmonary tuberculosis infection. What is the value of using the BACTEC MGIT960 culture system in a mouse model of tuberculosis infection?

　　Method: The experiment was divided into BACTEC MGIT960 culture group and LJ culture group. 36 C57BL/6 women were injected with 1.0×106 CFU/infection through the tail vein. In mLH37Rv bacterial solution mice, 18 mice were treated with rifampicin for 4 weeks after infection, 18 mice were injected with the same amount of phosphate buffered saline (PBS) as a control, and 36 mice were injected with BACTEC MGIT960 rapid culture and Roche to cultivate. Lung, spleen, spleen. Do you want to culture liver tissue homogenate?

　　Result: When using the BACTECMGIT960 culture method, is the positive time of liver, lung, and spleen tissue culture in the RIF treatment group (187.11±10.20) h? (187.11 ± 10.20) hours? (347.22 ± 12.70) hours? (276.39)±13.09)h, the control group is (142.50±11.70)h? (251.67 ± 16.63) hours? (230.28±7.22)h, there is a significant difference between the groups (Pu003c0.001); Roche culture, RIF treatment group liver, lung, spleen tissue bacterial mass (5.15±0.15) log10CFU? (3.30 ± 0.23) log10CFU? (3.40±0.25)log10CFU, the control group (5.90±0.25)log10CFU? (3.88 ± 0.31)) log10CFU? (4.15±0.30) log10CFU, is there a significant difference between groups (Pu003c0.001)?

　　Conclusion: BACTEC MGIT960 culture and Roche culture test the bacterial count, the culture results are the same, but the former test positive time is reduced by an average of half, and the degree of discrimination is also high, indicating that the BACTEC MGIT960 culture method has more advantages: detecting the bacterial load in the animal model. Can it be used to detect the bacterial load in an animal model of tuberculosis infection?