动物造模,小鼠模型 z-bio 2021-09-19 848

　　Objective: How to establish an experimental autoimmune myasthenia gravis animal model that meets international preclinical experimental standards?

　　Method: After the improvement of the method reported in the literature, the acetylcholine receptor (acetylcholine receptor, AchR) was extracted from the light, and the pure protein and SDS gel electrophoresis protein were qualitatively identified. The BCA method was used for protein quantification; for C57BL/6 mice The purified protein is actively immunized, a total of 3 immunizations (1 to 30 days respectively)? Day 60), are you clinically scoring EAMG mice? weight? What is the serum AchR antibody content? New stigmin test? Comprehensive evaluation such as electromyography?

　　Results: Compared with the adjuvant group, the EAMG model group began to develop symptoms after the third week, and the average clinical score increased significantly (Pu003c0.01); the weight of the affected mice was significantly (Pu003c0.01) Pu003c0.01); Ming test is positive; serum AchR antibody content is significantly increased (Pu003c0.01); EMG repeat EMG stimulation test is positive?

　　Conclusion: Was it extracted from the double-finned ray organ with black spots? Has the purified AchR protein successfully induced the C57BL/6EAMG mouse model, creating conditions for further research on myasthenia gravis?